rabbit mouse anti tom20  (Santa Cruz Biotechnology)

Journal: Cell Communication and Signaling : CCS

Article Title: C-terminal tail of MAVS dictates organelle targeting and innate immune response

doi: 10.1186/s12964-026-02753-y

Figure Lengend Snippet: Tail charge of MAVS modulates MAVS localization. A Multiple sequence alignment of MAVS C-terminal tail in Homo sapiens, Macaca mulatta, Equus caballus and Canis lupus, highlighted regions are arginine-rich stretches at MAVS C-terminal tail. B Schematic diagram of MAVS WT and its tail charge mutants to be employed in this study. C Type I IFN response does not affect peroxisomal sorting of MAVS. The number of MAVS-positive PMP70-positive puncta was counted in GFP-MAVS WT cells infected with or without SeV ( n = 45 cells pooled from three independent experiments). Box plots represent the 5% and 95% quartiles, respectively. ns: not significant. D , F , H , J , L , N Representative Z-projected figures of GFP-MAVS WT, 6R and 0R cells immunostained with anti-TOM20 (red) or anti-PMP70 (red). Nuclei were stained with DAPI (blue). Scale bars: 2 μm (inset). Circles labelled in white in panel ( D ) indicate MAVS-positive TOM20-negative puncta, circles labelled in yellow indicate MAVS-negative TOM20-positive puncta. E , G , I , K , M , O Line profiles in blocks of 11 pixels in GFP-MAVS WT, 6R and 0R cells immunostained with anti-TOM20 or anti-PMP70 ( n = 45 cells pooled from three independent experiments)

Article Snippet: Mouse monoclonal anti-TOM20 (IF: 1:100) , Santa Cruz , sc-17764.

Techniques: Sequencing, Infection, Staining

Journal: PLOS One

Article Title: Characterizing mitochondrial phenotypes and MERCS in aged human skeletal muscle myoblasts

doi: 10.1371/journal.pone.0343604

Figure Lengend Snippet: (a) Results of oxygen consumption measurements using the Seahorse Flux Analyzer. Each point represents the mean ± S. D. deviation for young and old cells (n = 3 biological replicates), with 20,000 cells per measurement. (b) Results of mitochondrial morphology. The images are partial views of cells stained with TOM20 antibody, with the scale bar representing 10 μm. The graph shows the average mitochondrial size per cell measured from the images, presented as violin plots (n = 3 biological replicates; cells counted: young, 239, young+FCCP, 220, old, 264). p -values were calculated using Dunnett’s test with young as the control, **; p < 0.01, ***; p < 0.001. (c) Results of mitochondrial DNA quantification (n = 3 biological replicates; cells counted: young, 3, old, 3). The quantity of mitochondrial genes was normalized using bAct as an internal standard. q-values were calculated using Welch’s t-test for multiple comparisons.

Article Snippet: The cells were then permeabilized for 10 min with 0.2% Triton and blocked by incubation with Blocking One Histo (Nacalai) for 1 h. After blocking, the cells were incubated for 1 h at RT with mouse monoclonal anti-TOM20 antibody (ab56783; 1:40 in PBS-T with 5% Blocking One Histo).

Techniques: Staining, Control